Recombinant Mouse 5'-nucleotidase (Nt5e), partial (Active), Mus musculus (Mouse), Mammalian cell, C-terminal 6xHis-tagged, 58.8 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse 5′-nucleotidase (Nt5e), partial (Active)

Recombinant Mouse 5′-nucleotidase (Nt5e), partial (Active) — Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Purity >95%.

SKU: BCACP-001265 Category:
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Product SKUBCACP-001265
Product DescriptionRecombinant Mouse 5'-nucleotidase (Nt5e) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 29-550aa. [Accession | Q61503].
Uniprot No.Q61503
Gene NamesNt5e
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region29-550aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight58.8 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human 4-1BBL-His at 10μg/ml can bind Human 4-1BB-Fc, the ED50 of Human 4-1BB-Fc is 16.8 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesNt5e; Nt5; Nte5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD antigen CD73

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Hydrolyzes IMP. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms.

Product Specifications

Recombinant Mouse 5'-nucleotidase (Nt5e), partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 29-550aa, with C-terminal 6xHis-tagged tag, molecular weight 58.8 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human 4-1BBL-His at 10μg/ml can bind Human 4-1BB-Fc, the ED50 of Human 4-1BB-Fc is 16.8 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:8224905). Hydrolyzes IMP (PubMed:8224905). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity)

Gene: Nt5e  |  Organism: Mus musculus

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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