Recombinant Human 5'-nucleotidase (NT5E) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 60.2 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human 5′-nucleotidase (NT5E) (Active)

Recombinant Human 5′-nucleotidase (NT5E) (Active) — Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Purity >95%.

SKU: BCACP-000099 Category:
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Product SKUBCACP-000099
Product DescriptionRecombinant Human 5'-nucleotidase (NT5E) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 27-549aa. [Accession | P21589].
Uniprot No.P21589
Gene NamesNT5E
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region27-549aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight60.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNFSF11 ) at 10 μg/ml can bind human TNFRSF11B, the EC50 is 2.651-7.646 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(5'-NT)(Ecto-5'-nucleotidase)(CD73)(NT5)(NTE)

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)
Disease Association: Calcification of joints and arteries (CALJA) : A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human 5'-nucleotidase (NT5E) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 27-549aa, with C-terminal 6xHis-tagged tag, molecular weight 60.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor)

Disease Association

Calcification of joints and arteries (CALJA) : A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homodimer

Gene: NT5E  |  Organism: Homo sapiens  |  Synonyms: 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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