Recombinant Human 5'-nucleotidase (NT5E) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 60.2 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human 5′-nucleotidase (NT5E) (Active)

Recombinant Human 5′-nucleotidase (NT5E) (Active) — Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Purity >95%.

SKU: BCACP-000099 Category:
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Product SKUBCACP-000099
Product DescriptionRecombinant Human 5'-nucleotidase (NT5E) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 27-549aa. [Accession | P21589].
Uniprot No.P21589
Gene NamesNT5E
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region27-549aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight60.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNFSF11 ) at 10 μg/ml can bind human TNFRSF11B, the EC50 is 2.651-7.646 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(5'-NT)(Ecto-5'-nucleotidase)(CD73)(NT5)(NTE)

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)
Disease Association: Calcification of joints and arteries (CALJA) : A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human 5'-nucleotidase (NT5E) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 27-549aa, with C-terminal 6xHis-tagged tag, molecular weight 60.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized TNFSF11 ) at 10 μg/ml can bind human TNFRSF11B, the EC50 is 2.651-7.646 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor)

Disease Association

Calcification of joints and arteries (CALJA) : A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homodimer

Gene: NT5E  |  Organism: Homo sapiens  |  Synonyms: 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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