Recombinant Mouse C-C chemokine receptor type 2 (Ccr2) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 44.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse C-C chemokine receptor type 2 (Ccr2) Protein

Recombinant Mouse C-C chemokine receptor type 2 (Ccr2) Protein — Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines. Purity >90%.

SKU: BCRECP-00218TM Categories: ,
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Product Specifications

Uniprot No.P51683
Gene NamesCcr2
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-373aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight44.3kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion. Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Epressed in mature thymocytes. Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines

Product Specifications

Recombinant Mouse C-C chemokine receptor type 2 (Ccr2) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-373aa, with N-terminal 10xHis-tagged tag, molecular weight 44.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines (PubMed:8631787, PubMed:8662823, PubMed:8996246). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (PubMed:28507030). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (PubMed:29930553). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed:29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed:29993042). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (PubMed:24806994, PubMed:29632244)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Epressed in mature thymocytes (PubMed:29930553). Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines (PubMed:8631787)

Subunit

Interacts with ARRB1 (By similarity). Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2 (By similarity). Interacts with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling (By similarity). Interacts with GNAI1 (By similarity)

Gene: Ccr2  |  Organism: Mus musculus  |  Synonyms: JE/FIC receptor; MCP-1 receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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