Recombinant Mouse C-C motif chemokine 27 protein (Ccl27) (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 10.9 kDa, purity >>98% as determined by SDS-PAGE.
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Recombinant Mouse C-C motif chemokine 27 protein (Ccl27) (Active)

Recombinant Mouse C-C motif chemokine 27 protein (Ccl27) (Active) — Chemotactic factor that attracts skin-associated memory T-lymphocytes. Purity >98%.

SKU: BCACP-000812 Categories: ,
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Product SKUBCACP-000812
Product DescriptionRecombinant Mouse C-C motif chemokine 27 protein (Ccl27) protein is expressed from E.Coli with Tag-Free. It contains 26-120aa. [Accession | Q9Z1X0].
Uniprot No.Q9Z1X0
Gene NamesCcl27
Purity>98% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region26-120aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight10.9 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCcl27; Ilc; Scya27C-C motif chemokine 27; CC chemokine ILC; Cutaneous T-cell-attracting chemokine; CTACK; ESkine; IL-11 R-alpha-locus chemokine; ALP; mILC; Skinkine; Small-inducible cytokine A27

Function

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Isoform 1 is predominantly expressed in placenta and weakly in skin. Isoform 2 is predominantly expressed in testes and brain, weakly in kidney and liver and even lower in heart and muscle. Low expression of both isoforms in other tissues

Product Specifications

Recombinant Mouse C-C motif chemokine 27 protein (Ccl27) (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 26-120aa, with Tag-Free tag, molecular weight 10.9 kDa, purity >98% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10

Subcellular Location

Nucleus

Tissue Specificity

Isoform 1 is predominantly expressed in placenta and weakly in skin. Isoform 2 is predominantly expressed in testes and brain, weakly in kidney and liver and even lower in heart and muscle. Low expression of both isoforms in other tissues

Subunit

Monomer, dimer, and tetramer. Heparin avidly promotes oligomerization. Interacts with TNFAIP6 (via Link domain)

Gene: Ccl27  |  Organism: Mus musculus  |  Synonyms: CC chemokine ILC; Cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; Skinkine; Small-inducible cytokine A27
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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