Recombinant Mouse C-C motif chemokine 5 protein (Ccl5), Mus musculus (Mouse), E.Coli, Tag-Free, 7.9 kDa, purity >>97% as determined by SDS-PAGE.
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Recombinant Mouse C-C motif chemokine 5 protein (Ccl5)

Recombinant Mouse C-C motif chemokine 5 protein (Ccl5) — Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Purity >97%.

SKU: BCACP-000798 Categories: ,
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Product SKUBCACP-000798
Product DescriptionRecombinant Mouse C-C motif chemokine 5 protein (Ccl5) protein is expressed from E.Coli with Tag-Free. It contains 24-91aa. [Accession | P30882].
Uniprot No.P30882
Gene NamesCcl5
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region24-91aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight7.9 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCcl5; Scya5C-C motif chemokine 5; MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: T-cell and macrophage specific

Product Specifications

Recombinant Mouse C-C motif chemokine 5 protein (Ccl5) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 24-91aa, with Tag-Free tag, molecular weight 7.9 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells

Subcellular Location

Secreted

Tissue Specificity

T-cell and macrophage specific

Gene: Ccl5  |  Organism: Mus musculus  |  Synonyms: MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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