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Recombinant Mouse C-X-C motif chemokine 14 protein (Cxcl14)

Recombinant Mouse C-X-C motif chemokine 14 protein (Cxcl14) — recombinant protein from Mus musculus (Mouse), expressed in E.Coli. Purity >95%.

SKU: BCACP-000793 Categories: ,
Product SKUBCACP-000793
Product DescriptionRecombinant Mouse C-X-C motif chemokine 14 protein (Cxcl14) protein is expressed from E.Coli with Tag-Free. It contains 23-99aa. [Accession | Q9WUQ5].
Uniprot No.Q9WUQ5
Gene NamesCxcl14
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region23-99aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight9.4 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/ml, corresponding to a specific activity of >2000 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCxcl14; Bmac; Kec; Ks1; Mip2g; Scyb14C-X-C motif chemokine 14; B-cell and monocyte-activating chemokine; Chemokine BRAK; Kidney-expressed chemokine CXC; MIP-2G; Small-inducible cytokine B14

Function

Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Highly expressed in brain, lung, ovary, muscle and in kidney and liver parenchyma, and at lower levels in bone marrow

Product Specifications

Recombinant Mouse C-X-C motif chemokine 14 protein (Cxcl14) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 23-99aa, with Tag-Free tag, molecular weight 9.4 kDa, purity >95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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