Recombinant Mouse Complement factor I (Cfi), Mus musculus (Mouse), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 69.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Complement factor I (Cfi)

Recombinant Mouse Complement factor I (Cfi) — Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Purity >85%.

SKU: BCRECP-002358 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-002358
Product DescriptionRecombinant Mouse Complement factor I (Cfi) Protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 19-603aa. [Accession | Q61129].
Uniprot No.Q61129
Gene NamesCfi
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-603aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight69.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces.

Biological Context

Subcellular Location: Secreted, extracellular space
Tissue Specificity: Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes

Product Specifications

Recombinant Mouse Complement factor I (Cfi) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 19-603aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 69.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization

Subcellular Location

Secreted, extracellular space

Tissue Specificity

Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes

Subunit

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site. Interacts with C3b. Interacts with complement factor H

Gene: Cfi  |  Organism: Mus musculus  |  Synonyms: C3B/C4B inactivator
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top