Recombinant Mouse Cytotoxic and regulatory T-cell molecule (Crtam), partial (Active)

Function

Mediates heterophilic cell-cell adhesion which regulates the activation, differentiation and tissue retention of various T-cell subsets (PubMed:15811952, PubMed:18329370, PubMed:19752223, PubMed:24687959). Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed:15811952). Regulates CD8+ T-cell proliferation in response to T-cell receptor (TCR) activation (PubMed:18329370). Appears to be dispensable for CD8+ T-cell-mediated cytotoxicity (PubMed:19752223). Interaction with SCRIB promotes the late phase of cellular polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG, IL17 and IL22 production (PubMed:18329370). By interacting with CADM1 on CD8+ dendritic cells, regulates the retention of activated CD8+ T-cells within the draining lymph node (PubMed:19752223). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (PubMed:24687959). Interaction with CADM1 promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (PubMed:24687959)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT, NK and CD8+ T-cells (at protein level) (PubMed:10811014, PubMed:15811952, PubMed:18329370, PubMed:19752223). Transiently expressed in activated CD8+ T-cells and a subset of activated CD4+ T-cells (at protein level) (PubMed:18329370). Expressed in activated intestinal T-cells, specifically intraepithelial CD4+ CD8+ T-cells, intraepithelial CD4+ T-cells and, CD8+ T-cells in the intestine epithelium, lamina propria, Peyer's Patches and mesenteric lymph nodes (PubMed:24687959). Also expressed in spleen, brain and testis (PubMed:10811014)

Subunit

Monomer (By similarity). May form homodimer (via Ig-like V-type domain) (By similarity). Interacts (via Ig-like V-type domain) with CADM1 (via Ig-like V-type domain); the interaction competes with CRTAM homodimerization and CADM1 homodimerization (PubMed:15781451, PubMed:15811952). Interacts (via PDZ-binding motif) with SCRIB (via PDZ domain 3); the interaction promotes CRTAM and SCRIB polarization in a subset of CD4+ T-cells (PubMed:18329370)

• Kennedy J. et al. A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM). — J. Leukoc. Biol. (2000)
• Carninci P. et al. The transcriptional landscape of the mammalian genome. — Science (2005)
• Church D.M. et al. Lineage-specific biology revealed by a finished genome assembly of the mouse. — PLoS Biol. (2009)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Boles K.S. et al. The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM. — Blood (2005)
• Galibert L. et al. Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule. — J. Biol. Chem. (2005)
• Yeh J.H. et al. Regulation of a late phase of T cell polarity and effector functions by Crtam. — Cell (2008)
• Takeuchi A. et al. CRTAM confers late-stage activation of CD8+ T cells to regulate retention within lymph node. — J. Immunol. (2009)