Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 20.0 kDa, purity >>98% as determined by SDS­PAGE.

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active)

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active) — The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Purity >98%.

SKU: BCACP-000877 Category:
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Product SKUBCACP-000877
Product DescriptionRecombinant Mouse Desert hedgehog protein (Dhh) protein is expressed from E.Coli with Tag-Free. It contains 24-198aa. [Accession | Q61488].
Uniprot No.Q61488
Gene NamesDhh
Purity>98% as determined by SDS­PAGE.
Expression SystemE.Coli
Expression Region24-198aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight20.0 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesDhhDesert hedgehog protein; DHH; HHG-3) [Cleaved into: Desert hedgehog protein N-product; Desert hedgehog protein C-product]

Function

The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of DHH, patched represses the constitutive signaling activity of SMO.

Biological Context

Subcellular Location: Secreted; Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in adult testes. Not expressed in limb buds

Product Specifications

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 24-198aa, with Tag-Free tag, molecular weight 20.0 kDa, purity >98% as determined by SDS­PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development (PubMed:40494346). Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes (PubMed:33063110, PubMed:40494346, PubMed:9811851). In the absence of DHH, patched represses the constitutive signaling activity of SMO (By similarity). DHH plays a key role in male gonad development (spermatogenesis) and the formation of Schwann cells (PubMed:10482238, PubMed:11090455, PubMed:8805249). Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules (PubMed:11090455, PubMed:8805249). In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube (PubMed:10482238). May induce motor neurons in lateral neural tube and may have a polarizing activity (By similarity). Involved in tissue regeneration: secreted from epithelial neuroendocrine cells and elicits a regenerative response from mesenchymal cells (PubMed:40494346). Functions in cell-cell mediated juxtacrine signaling (By similarity). Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism (PubMed:32151560). Promotes endothelium integrity (PubMed:33063110)

Subcellular Location

Secreted; Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in adult testes (PubMed:8805249). Not expressed in limb buds

Subunit

Multimer

Gene: Dhh  |  Organism: Mus musculus  |  Synonyms: HHG-3
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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