Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 6xHis-tagged, 32.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5) Protein

Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5) Protein — Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission and as an important regulat… Purity >85%.

SKU: BCRECP-00356TM Categories: ,
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Product Specifications

Uniprot No.Q3KNM2
Gene NamesMarchf5
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-278aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight32.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission and as an important regulator of immune response. Plays a crucial role in maintaining mitochondrial homeostasis by regulating the dynamics of mitochondria through the ubiquitination of key proteins involved in fission and fusion such as FIS1, DNM1L and MFN1. Acts as a critical determinant of mitotic apoptosis through both MCL1-dependent and -independent pathways.

Biological Context

Subcellular Location: Mitochondrion outer membrane (Multi-pass membrane protein); Endoplasmic reticulum membrane (Multi-pass membrane protein)
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Mouse E3 ubiquitin-protein ligase MARCH5 (Marchf5) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-278aa, with N-terminal 6xHis-tagged tag, molecular weight 32.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not Test

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission and as an important regulator of immune response. Plays a crucial role in maintaining mitochondrial homeostasis by regulating the dynamics of mitochondria through the ubiquitination of key proteins involved in fission and fusion such as FIS1, DNM1L and MFN1 (By similarity). Acts as a critical determinant of mitotic apoptosis through both MCL1-dependent and -independent pathways (PubMed:36329234). Turns off persistent immune signaling by degrading oligomeric complexes of retinoic acid-inducible gene I/DDX58 and mitochondrial antiviral-signaling protein/MAVS formed upon RNA virus infection. Promotes STING-mediated type-I interferon production via 'Lys-63'-linked ubiquitination of STING1 thereby preserving its activity and preventing the formation of inactive STING1 polymers. Plays also an essential role in the formation of PEX3-containing vesicles in the de novo biogenesis of peroxisomes from mitochondria. Acts as a regulator of NLRP3 inflammasome activation on the mitochondria by mediating the 'Lys-27'-linked polyubiquitination of NLRP3, positively regulating the NLRP3-NEK7 complex formation and NLRP3 oligomerization (PubMed:37575012)

Subcellular Location

Mitochondrion outer membrane (Multi-pass membrane protein); Endoplasmic reticulum membrane (Multi-pass membrane protein)

Subunit

Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1

Pathway

Protein modification; protein ubiquitination

Gene: Marchf5  |  Organism: Mus musculus  |  Synonyms: Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; RING-type E3 ubiquitin transferase MARCHF5

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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