Recombinant Mouse Fibroblast growth factor 2 (Fgf2) (Active), Mus musculus (Mouse), E.coli, Tag-Free, 17.15 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Mouse Fibroblast growth factor 2 (Fgf2) (Active)

Recombinant Mouse Fibroblast growth factor 2 (Fgf2) (Active) — Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Purity >95%.

SKU: BCACP-001223 Categories: ,
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Product SKUBCACP-001223
Product DescriptionRecombinant Mouse Fibroblast growth factor 2 (Fgf2) protein is expressed from E.coli with Tag-Free. It contains 1-154aa. [Accession | P15655].
Uniprot No.P15655
Gene NamesFgf2
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-154aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight17.15 kDa
ActivityThe ED50 as determined by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells is 5-25 pg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesFgf2; Fgf-2Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

Function

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3.

Biological Context

Subcellular Location: Secreted; Nucleus

Product Specifications

Recombinant Mouse Fibroblast growth factor 2 (Fgf2) (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-154aa, with Tag-Free tag, molecular weight 17.15 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Also acts as an integrin ligand which is required for FGF2 signaling (By similarity). Binds to integrin ITGAV:ITGB3 (By similarity). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (By similarity). Functions as a potent mitogen in vitro (By similarity). Can induce angiogenesis (By similarity). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (By similarity)

Subcellular Location

Secreted; Nucleus

Subunit

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2 (By similarity). Interacts with FGFBP3 (PubMed:20851768). Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling (By similarity). Interacts with SNORC (via the extracellular domain) (PubMed:28323137). Interacts with glypican GPC3 (By similarity)

Gene: Fgf2  |  Organism: Mus musculus  |  Synonyms: Basic fibroblast growth factor; Heparin-binding growth factor 2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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