Recombinant Mouse Fibroblast growth factor 21 (Fgf21), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 23.9kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Fibroblast growth factor 21 (Fgf21)

Recombinant Mouse Fibroblast growth factor 21 (Fgf21) — Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Purity >90%.

SKU: BCRECP-002730 Categories: ,
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Product Specifications

Product SkuBCRECP-002730
Product DescriptionRecombinant Mouse Fibroblast growth factor 21 (Fgf21) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 29-210aa. [Accession | Q9JJN1].
Uniprot No.Q9JJN1
Gene NamesFgf21
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region29-210aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight23.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Most abundantly expressed in the liver, also expressed in the thymus at lower levels. Expressed in skeletal muscle (at protein level). Secreted in plasma (at protein level)

Product Specifications

Recombinant Mouse Fibroblast growth factor 21 (Fgf21) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 29-210aa, with N-terminal 6xHis-tagged tag, molecular weight 23.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity

Subcellular Location

Secreted

Tissue Specificity

Most abundantly expressed in the liver, also expressed in the thymus at lower levels (PubMed:10858549, PubMed:30389664). Expressed in skeletal muscle (at protein level). Secreted in plasma (at protein level) (PubMed:30605666)

Subunit

Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4 (By similarity)

Gene: Fgf21  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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