Recombinant Mouse Gap junction alpha-1 protein (Gja1), partial, Mus musculus (Mouse), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 21.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Gap junction alpha-1 protein (Gja1), partial

Recombinant Mouse Gap junction alpha-1 protein (Gja1), partial — recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell. Purity >90%.

SKU: BCRECP-002860 Category:
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Product Specifications

Product SkuBCRECP-002860
Product DescriptionRecombinant Mouse Gap junction alpha-1 protein (Gja1) Protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 232-382aa. [Accession | P23242].
Uniprot No.P23242
Gene NamesGja1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region232-382aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight21.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Connexin 43 is possibly the ATP-induced pore of mouse macrophages.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell junction, gap junction; Endoplasmic reticulum; Cell junction
Tissue Specificity: Expressed at intercalated disks in cardiomyocytes (at protein level). Expressed in bladder smooth muscle cells (at protein level). Expressed in astrocytes (at protein level). Expressed in non-sensory epithelial cells, and in fibrocytes of the spiral ligament and the spiral limbus

Product Specifications

Recombinant Mouse Gap junction alpha-1 protein (Gja1), partial is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 232-382aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 21.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Connexin 43 is possibly the ATP-induced pore of mouse macrophages

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell junction, gap junction; Endoplasmic reticulum; Cell junction

Tissue Specificity

Expressed at intercalated disks in cardiomyocytes (at protein level) (PubMed:26085008, PubMed:29959233). Expressed in bladder smooth muscle cells (at protein level). Expressed in astrocytes (at protein level) (PubMed:15213231). Expressed in non-sensory epithelial cells, and in fibrocytes of the spiral ligament and the spiral limbus

Subunit

A connexon/hemichannel is composed of a hexamer of connexins (By similarity). Forms functional homotypic gap junctions consisting of identical hemichannels both made of the same connexin type as well as heterotypic gap junctions consisting of two different hemichannels, each containing a different type of connexin (By similarity). Assembles with GJC1 to form heterotypic gap junction channels (By similarity). Interacts with CSNK1D (By similarity). Interacts with RIC1/CIP150 (By similarity). Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain). Interacts with CNST. Interacts with SGSM3. Interacts with NOV (By similarity). Interacts with TMEM65 (PubMed:26403541). Interacts with ANK3/ANKG and PKP2 (By similarity)

Gene: Gja1  |  Organism: Mus musculus  |  Synonyms: Connexin-43; Gap junction 43 kDa heart protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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