Recombinant Mouse Interleukin-2 (Il2) (Active)

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:14614860, PubMed:9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:14614860, PubMed:27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed:14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed:9814585)

Subcellular Location

Secreted

Tissue Specificity

Produced by immune cells including dendritic cells. In contrast, macrophages do not produce IL2 upon bacterial stimulation

• Fuse A. et al. Organization and structure of the mouse interleukin-2 gene. — Nucleic Acids Res. (1984)
• Kashima N. et al. Unique structure of murine interleukin-2 as deduced from cloned cDNAs. — Nature (1985)
• Yokota T. et al. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. — Proc. Natl. Acad. Sci. U.S.A. (1985)
• Degrave W. et al. Cloning and structure of a mouse interleukin-2 chromosomal gene. — Mol. Biol. Rep. (1986)
• Lyons P.A. et al. Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping. — Genome Res. (2000)
• Ma R.Z. et al. PubMed: CI-FJPL5KHL54K7O (MAY-)
• Church D.M. et al. Lineage-specific biology revealed by a finished genome assembly of the mouse. — PLoS Biol. (2009)
• Matesanz F. et al. A new cDNA sequence for the murine interleukin-2 gene. — Biochim. Biophys. Acta (1992)