Recombinant Mouse Interleukin-33 (Il33), partial (Active), Mus musculus (Mouse), E.coli, Tag-Free, 17.6 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Mouse Interleukin-33 (Il33), partial (Active)

Recombinant Mouse Interleukin-33 (Il33), partial (Active) — In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester … Purity >95%.

SKU: BCACP-001259 Categories: ,
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Product SKUBCACP-001259
Product DescriptionRecombinant Mouse Interleukin-33 (Il33) protein is expressed from E.coli with Tag-Free. It contains 109-266aa. [Accession | Q8BVZ5].
Uniprot No.Q8BVZ5
Gene NamesIl33
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region109-266aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight17.6 kDa
ActivityThe ED50 as determined by its ability to bind Human Galectin-3 in functional ELISA is less than 20 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl33Interleukin-33; IL-33) [Cleaved into: Interleukin-33(102-266); Interleukin-33(109-266)]

Function

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Mouse Interleukin-33 (Il33), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 109-266aa, with Tag-Free tag, molecular weight 17.6 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (By similarity). This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity)

Subcellular Location

Nucleus

Subunit

Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion

Gene: Il33  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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