Recombinant Mouse Interleukin-4 (Il4) (Active)

Function

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:3083412). Induces the expression of class II MHC molecules on resting B-cells (PubMed:3498301). Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:3498301). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (By similarity). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (PubMed:26416964). In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (PubMed:25772794, PubMed:28202615). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:25847241, PubMed:8624821)

Subcellular Location

Secreted

Subunit

Interacts with IL4R. Interacts with IL13RA1

• Noma Y. et al. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter. — Nature (1986)
• Otsuka T. et al. Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities. — Nucleic Acids Res. (1987)
• Lee F. et al. Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities. — Proc. Natl. Acad. Sci. U.S.A. (1986)
• Sideras P. et al. IgG1 induction factor: a single molecular entity with multiple biological functions. — Adv. Exp. Med. Biol. (1987)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Carr C. et al. Disulfide assignments in recombinant mouse and human interleukin 4. — Biochemistry (1991)
• Kaplan M.H. et al. Stat6 is required for mediating responses to IL-4 and for development of Th2 cells. — Immunity (1996)
• Edukulla R. et al. Th2 Cytokines Augment IL-31/IL-31RA Interactions via STAT6-dependent IL-31RA Expression. — J. Biol. Chem. (2015)