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Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1)

Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >90%.

SKU: BCRECP-003266 Category:
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Product Specifications

Product SkuBCRECP-003266
Product DescriptionRecombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) Protein is expressed from E.coli with Tag-Free. It contains 2-325aa. [Accession | Q99JW4].
Uniprot No.Q99JW4
Gene NamesLims1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-325aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight37.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

Biological Context

Subcellular Location: Cell junction, focal adhesion; Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 2-325aa, with Tag-Free tag, molecular weight 37.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration

Subcellular Location

Cell junction, focal adhesion; Cell membrane (Peripheral membrane protein)

Subunit

Component of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex composed of ILK, LIMS1/PINCH and PARVA; the complex binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling (By similarity). Formation of the IPP complex is dependent on protein kinase C and precedes integrin-mediated cell adhesion and spreading (By similarity). Competes with LIMS2 for interaction with ILK. Interacts with SH3/SH2 adapter NCK2, thereby linking the complex to cell surface receptors (By similarity). Interacts (via LIM zinc-binding 5) with TGFB1I1

Gene: Lims1  |  Organism: Mus musculus  |  Synonyms: Particularly interesting new Cys-His protein 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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