Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1), Mus musculus (Mouse), Baculovirus, N-terminal 10xHis-tagged, 39.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1)

Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) — recombinant protein from Mus musculus (Mouse), expressed in Baculovirus. Purity >90%.

SKU: BCRECP-003265 Category:
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Product Specifications

Product SkuBCRECP-003265
Product DescriptionRecombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged. It contains 2-325aa. [Accession | Q99JW4].
Uniprot No.Q99JW4
Gene NamesLims1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region2-325aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight39.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

Biological Context

Subcellular Location: Cell junction, focal adhesion; Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1 (Lims1) is a recombinant protein from Mus musculus (Mouse), expressed in Baculovirus, covering amino acids 2-325aa, with N-terminal 10xHis-tagged tag, molecular weight 39.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration

Subcellular Location

Cell junction, focal adhesion; Cell membrane (Peripheral membrane protein)

Subunit

Component of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex composed of ILK, LIMS1/PINCH and PARVA; the complex binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling (By similarity). Formation of the IPP complex is dependent on protein kinase C and precedes integrin-mediated cell adhesion and spreading (By similarity). Competes with LIMS2 for interaction with ILK. Interacts with SH3/SH2 adapter NCK2, thereby linking the complex to cell surface receptors (By similarity). Interacts (via LIM zinc-binding 5) with TGFB1I1

Gene: Lims1  |  Organism: Mus musculus  |  Synonyms: Particularly interesting new Cys-His protein 1

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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