Recombinant Mouse Otoferlin (Otof), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 54.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Otoferlin (Otof), partial

Recombinant Mouse Otoferlin (Otof), partial — Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Purity >85%.

SKU: BCRECP-003983 Category:
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Product Specifications

Product SkuBCRECP-003983
Product DescriptionRecombinant Mouse Otoferlin (Otof) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1461-1868aa. [Accession | Q9ESF1].
Uniprot No.Q9ESF1
Gene NamesOtof
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1461-1868aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight54.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs).

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass type IV membrane protein); Basolateral cell membrane (Single-pass type IV membrane protein); Endoplasmic reticulum membrane (Single-pass type IV membrane protein); Golgi apparatus membrane (Single-pass type IV membrane protein); Presynaptic cell membrane (Single-pass type IV membrane protein); Cell membrane (Single-pass type IV membrane protein); Golgi apparatus, trans-Golgi network
Tissue Specificity: Isoform 1 is expressed in cochlea and brain. Expressed in the cochlear and vestibular hair cells. Expressed in both inner and outer hair cells (IHCs and OHCs) and cochlear ganglions neurons at postnatal day 2 (P2) and 6 (P6). Expressed only in IHCs at postnatal day 60 (P60) (at protein level). Strongly expressed in brain and inner ear. In the inner ear, it is mainly expressed in the cochlear IHC and vestibular type I sensory hair cells. Weakly expressed in eye, heart, skeletal muscle, liver, kidney, lung and testis

Product Specifications

Recombinant Mouse Otoferlin (Otof), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1461-1868aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 54.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass type IV membrane protein); Basolateral cell membrane (Single-pass type IV membrane protein); Endoplasmic reticulum membrane (Single-pass type IV membrane protein); Golgi apparatus membrane (Single-pass type IV membrane protein); Presynaptic cell membrane (Single-pass type IV membrane protein); Cell membrane (Single-pass type IV membrane protein); Golgi apparatus, trans-Golgi network

Tissue Specificity

Isoform 1 is expressed in cochlea and brain. Expressed in the cochlear and vestibular hair cells. Expressed in both inner and outer hair cells (IHCs and OHCs) and cochlear ganglions neurons at postnatal day 2 (P2) and 6 (P6). Expressed only in IHCs at postnatal day 60 (P60) (at protein level). Strongly expressed in brain and inner ear. In the inner ear, it is mainly expressed in the cochlear IHC and vestibular type I sensory hair cells. Weakly expressed in eye, heart, skeletal muscle, liver, kidney, lung and testis

Subunit

Interacts with SNAP25; the interaction is direct. Interacts with STX1; the interaction is direct. Interacts with RAB8B

Gene: Otof  |  Organism: Mus musculus  |  Synonyms: Fer-1-like protein 2; Protein pachanga
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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