Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp), Mus musculus (Mouse), E.coli, N-terminal 6xHis-SUMO-tagged, 47.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp)

Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp) — Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5′-phosphate (PNP) and pyridoxamine 5′-phosphate (PMP), with order of substrate prefer… Purity >90%.

SKU: BCRECP-004045 Categories: ,
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Product Specifications

Product SkuBCRECP-004045
Product DescriptionRecombinant Mouse Pyridoxal phosphate phosphatase (Pdxp) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-292aa. [Accession | P60487].
Uniprot No.P60487
Gene NamesPdxp
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-292aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight47.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Cell projection, ruffle membrane (Peripheral membrane protein); Cell projection, lamellipodium membrane (Peripheral membrane protein); Cell membrane (Peripheral membrane protein)
Tissue Specificity: Ubiquitous. highly expressed in brain (at protein level)

Product Specifications

Recombinant Mouse Pyridoxal phosphate phosphatase (Pdxp) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-292aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 47.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism (PubMed:24338473, PubMed:24338687, PubMed:25783190). Also functions as a protein serine phosphatase that specifically dephosphorylates 'Ser-3' in proteins of the actin-depolymerizing factor (ADF)/cofilin family like CFL1 and DSTN. Thereby, regulates cofilin-dependent actin cytoskeleton reorganization, being required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phosphothreonines in LIMK1. Does not dephosphorylate peptides containing phosphotyrosine (By similarity)

Subcellular Location

Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Cell projection, ruffle membrane (Peripheral membrane protein); Cell projection, lamellipodium membrane (Peripheral membrane protein); Cell membrane (Peripheral membrane protein)

Tissue Specificity

Ubiquitous. highly expressed in brain (at protein level)

Subunit

Homodimer

Gene: Pdxp  |  Organism: Mus musculus  |  Synonyms: Pyridoxal 5'-phosphate phosphatase; Pyridoxal phosphate phosphatase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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