Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (Pdk3), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 30.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (Pdk3), partial

Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (Pdk3), partial — Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Purity >90%.

SKU: BCRECP-004044 Categories: , ,
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Product Specifications

Product SkuBCRECP-004044
Product DescriptionRecombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (Pdk3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 131-362aa. [Accession | Q922H2].
Uniprot No.Q922H2
Gene NamesPdk3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region131-362aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight30.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet.

Biological Context

Subcellular Location: Mitochondrion matrix

Product Specifications

Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (Pdk3), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 131-362aa, with N-terminal 6xHis-tagged tag, molecular weight 30.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species (By similarity)

Subcellular Location

Mitochondrion matrix

Subunit

Homodimer. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (By similarity)

Gene: Pdk3  |  Organism: Mus musculus  |  Synonyms: Pyruvate dehydrogenase kinase isoform 3

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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