Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 63.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2)

Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) — Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. Purity >85%.

SKU: BCRECP-002049 Categories: , ,
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Product Specifications

Product SkuBCRECP-002049
Product DescriptionRecombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-481aa. [Accession | Q60823].
Uniprot No.Q60823
Gene NamesAkt2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-481aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight63.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. May play a role in myoblast differentiation. In this context, may act through PITX2 phosphorylation.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Cell membrane (Peripheral membrane protein); Early endosome

Product Specifications

Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-481aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 63.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. May play a role in myoblast differentiation (PubMed:17565718). In this context, may act through PITX2 phosphorylation. Unphosphorylated PITX2 associates with an ELAVL1/HuR-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation (PubMed:20019746). Also involved in the negative regulation of myogenesis in response to stress conditions. In this context, acts by phosphorylating ANKRD2 (PubMed:21737686). May also be a key regulator of glucose uptake. Regulates insulin-stimulated glucose transport by the increase of glucose transporter GLUT4 translocation from intracellular stores to the plasma membrane. In this context, acts by phosphorylating C2CD5/CDP138 on 'Ser-197' in insulin-stimulated adipocytes (PubMed:21907143). Through the phosphorylation of CLK2 on 'Thr-343', involved in insulin-regulated suppression of hepatic gluconeogenesis (PubMed:20074525)

Subcellular Location

Cytoplasm; Nucleus; Cell membrane (Peripheral membrane protein); Early endosome

Subunit

Interacts with BTBD10 (PubMed:18160256). Interacts with KCTD20 (PubMed:24156551). Interacts (via PH domain) with MTCP1, TCL1A and TCL1B; this interaction may facilitate AKT2 oligomerization and phosphorylation, hence increasing kinase activity (By similarity). Interacts with PHB2; this interaction may be important for myogenic differentiation (PubMed:17565718). Interacts (when phosphorylated) with CLIP3/ClipR-59; this interaction promotes cell membrane localization (PubMed:19139280). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529, PubMed:20189988)

Gene: Akt2  |  Organism: Mus musculus  |  Synonyms: Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta
Key Publications

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