Recombinant Mouse Sodium/potassium-transporting ATPase subunit beta-1 (Atp1b1) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 36.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Sodium/potassium-transporting ATPase subunit beta-1 (Atp1b1) Protein

Recombinant Mouse Sodium/potassium-transporting ATPase subunit beta-1 (Atp1b1) Protein — recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system. Purity >85%.

SKU: BCRECP-00248TM Category:
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Product Specifications

Uniprot No.P14094
Gene NamesAtp1b1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-304aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight36.7kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Involved in cell adhesion and establishing epithelial cell polarity.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein); Apical cell membrane (Single-pass type II membrane protein); Cell membrane, sarcolemma
Tissue Specificity: Expressed in cardiac muscle and in flexor digitorum brevis (FDB) muscle (at protein level). Expressed in a circadian manner in the kidney and aorta (at protein level)

Product Specifications

Recombinant Mouse Sodium/potassium-transporting ATPase subunit beta-1 (Atp1b1) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-304aa, with N-terminal 10xHis-tagged tag, molecular weight 36.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in cell adhesion and establishing epithelial cell polarity

Subcellular Location

Cell membrane (Single-pass type II membrane protein); Apical cell membrane (Single-pass type II membrane protein); Cell membrane, sarcolemma

Tissue Specificity

Expressed in cardiac muscle and in flexor digitorum brevis (FDB) muscle (at protein level) (PubMed:23392350). Expressed in a circadian manner in the kidney and aorta (at protein level) (PubMed:30012868)

Subunit

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with catalytic subunit ATP12A (By similarity). Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3 (PubMed:15743908). Interacts with NKAIN1, NKAIN2 and NKAIN4 (PubMed:17606467). Interacts with MLC1 (By similarity). Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM (By similarity). Interacts with KIRREL3 (By similarity). Interacts with OBSCN (via protein kinase domain 1) (PubMed:23392350)

Gene: Atp1b1  |  Organism: Mus musculus  |  Synonyms: Sodium/potassium-dependent ATPase subunit beta-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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