Recombinant Mouse Sphingosine 1-phosphate receptor 2 (S1pr2) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 44.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Sphingosine 1-phosphate receptor 2 (S1pr2) Protein

Recombinant Mouse Sphingosine 1-phosphate receptor 2 (S1pr2) Protein — Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). Purity >85%.

SKU: BCRECP-00200TM Category:
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Product Specifications

Uniprot No.P52592
Gene NamesS1pr2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-352aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight44.9kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues. Receptor for the chemokine-like protein FAM19A5.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Most abundant in heart and lung; low, but clearly observed in kidney, liver and thymus; much lower but detectable in brain, testis, stomach and intestine. Not significantly detected in any of the sections of embryonic day (E) 14-18, except in embryonic brain

Product Specifications

Recombinant Mouse Sphingosine 1-phosphate receptor 2 (S1pr2) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-352aa, with N-terminal 10xHis-tagged tag, molecular weight 44.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P) (By similarity). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues (By similarity). Receptor for the chemokine-like protein FAM19A5 (By similarity). Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration (By similarity). In lymphoid follicles, couples the binding of S1P to the activation of GNA13 and downstream inhibition of AKT activation leading to suppression of germinal center (GC) B cell growth and migration outside the GC niche

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Most abundant in heart and lung; low, but clearly observed in kidney, liver and thymus; much lower but detectable in brain, testis, stomach and intestine. Not significantly detected in any of the sections of embryonic day (E) 14-18, except in embryonic brain

Gene: S1pr2  |  Organism: Mus musculus  |  Synonyms: Endothelial differentiation G protein-coupled receptor 5; Lysophospholipid receptor B2; Sphingosine 1-phosphate receptor Edg-5
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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