Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, C-terminal 10xHis-tagged, 41.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2) Protein

Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2) Protein — Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). Purity >85%.

SKU: BCRECP-00198TM Category:
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Product Specifications

Uniprot No.P47752
Gene NamesS1pr2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-352aa
SpeciesRattus norvegicus (Rat)
Tag InfoC-terminal 10xHis-tagged
Molecular weight41.6kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues. Receptor for the chemokine-like protein FAM19A5.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in all developing tissues with highest levels detected in primitive, transformed cells. Relative abundance: lung > kidney = skin = gut > spleen > brain > liver

Product Specifications

Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-352aa, with C-terminal 10xHis-tagged tag, molecular weight 41.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P) (PubMed:10383399). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues (PubMed:10383399). Receptor for the chemokine-like protein FAM19A5 (PubMed:29453251). Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration (PubMed:29453251). In lymphoid follicles, couples the binding of S1P to the activation of GNA13 and downstream inhibition of AKT activation leading to suppression of germinal center (GC) B cell growth and migration outside the GC niche

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in all developing tissues with highest levels detected in primitive, transformed cells. Relative abundance: lung > kidney = skin = gut > spleen > brain > liver

Gene: S1pr2  |  Organism: Rattus norvegicus  |  Synonyms: AGR16; Endothelial differentiation G protein-coupled receptor 5; G protein-coupled receptor H218; Sphingosine 1-phosphate receptor Edg-5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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