Recombinant Mycobacterium tuberculosis 6 kDa early secretory antigenic target (esxA), partial, Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, C-terminal 6xHis-tagged, 16.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis 6 kDa early secretory antigenic target (esxA), partial

Recombinant Mycobacterium tuberculosis 6 kDa early secretory antigenic target (esxA), partial — May be critical in pro-bacteria versus pro-host interactions; ESX-1 mediates DNA mediated export (maybe via EsxA). Purity >90%.

SKU: BCRECP-002662 Category:
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Product Specifications

Product SkuBCRECP-002662
Product DescriptionRecombinant Mycobacterium tuberculosis 6 kDa early secretory antigenic target (esxA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 6-95aa. [Accession | P9WNK7].
Uniprot No.P9WNK7
Gene NamesesxA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region6-95aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoC-terminal 6xHis-tagged
Molecular weight16.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

May be critical in pro-bacteria versus pro-host interactions; ESX-1 mediates DNA mediated export (maybe via EsxA). The DNA interacts with host (human) cGAS, leading to cGAMP production and activation of the host STING-TBK-1-IRF-3 signaling pathway that leads to IFN-beta which is thought to be 'pro-bacteria'.

Product Specifications

Recombinant Mycobacterium tuberculosis 6 kDa early secretory antigenic target (esxA), partial — May be critical in pro-bacteria versus pro-host interactions; ESX-1 mediates DNA mediated export (maybe via EsxA). Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May be critical in pro-bacteria versus pro-host interactions; ESX-1 mediates DNA mediated export (maybe via EsxA). The DNA interacts with host (human) cGAS, leading to cGAMP production and activation of the host STING-TBK-1-IRF-3 signaling pathway that leads to IFN-beta which is thought to be 'pro-bacteria'. Mycobacterial dsDNA also interacts with AIM2-NLRP3-ASC to activate an inflammasome, leading to the 'pro-host' IL-1-beta (PubMed:26048136, PubMed:26048138)

Subcellular Location

Secreted; Secreted, cell wall; Host cell surface; Host cytoplasm; Host endoplasmic reticulum; Host cell membrane

Subunit

Forms a tight 1:1 complex with EsxB (CFP-10) (PubMed:11940590, PubMed:14557536, PubMed:15973432, PubMed:16048998, PubMed:16973880, PubMed:19854905, PubMed:19906174, PubMed:20085764, PubMed:23150662, PubMed:24586681, PubMed:26260636). The complex persists even after secretion (PubMed:16048998). Recombinant His-tagged protein forms dimers and higher order multimers; how the protein is prepared influences its multimerization and its subsequent properties in vitro (PubMed:26260636). In vitro EsxB only interacts with non-acetylated EsxA; it also interacts with C-terminally truncated EsxA (missing the last 10 residues) (PubMed:15378760). The native EsxA-EsxB complex dissociates at pH 4.0, and EsxA may then be freed to lyse (host) membranes (PubMed:17557817). Another study using recombinant protein did not find dissociation at acidic pH (PubMed:23150662). Recombinant heterodimer (with a His tag on EsxB) can be dissociated by the detergents amidosulfobetaine-14 and lauryldimethylamine N-oxide (PubMed:26260636). Interacts with EspF (PubMed:19682254). An artificial EsxB-EsxA heterodimer interacts with EspA, EccB1, EccCa1, EccCb1, EspI, EspJ, EccA2 and EccE2; the latter 2 are from the adjacent ESX-2 locus (PubMed:19854905). Contributes to the heterodimer's interaction with EccCb1 (PubMed:19854905). Interacts with host (mouse and human) TLR2 (PubMed:17486091, PubMed:20800577). Interacts with host (human) beta-2-microglobulin (B2M) alone and in complex with EsxB; only binds free B2M and not B2M in complex with HLA-I (PubMed:25356553). The EsxA-EsxB-B2M complex can be detected in the host endoplasmic reticulum (PubMed:25356553). The B2M-EsxA complex can be detected in patients with pleural tuberculosis and is stable from pH 4.0 to 8.0 and in the presence of 2M NaCl (PubMed:25356553)

Gene: esxA  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: ESAT-6
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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