Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 10xHis-tagged, 54.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125)

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) — Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Purity >85%.

SKU: BCRECP-002492 Category:
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Product Specifications

Product SkuBCRECP-002492
Product DescriptionRecombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-433aa. [Accession | P9WPP1].
Uniprot No.P9WPP1
Gene Namescyp125
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-433aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 10xHis-tagged
Molecular weight54.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.

Biological Context

Pathway: Steroid metabolism; cholesterol degradation

Product Specifications

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) is a recombinant protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), expressed in E.coli, covering amino acids 1-433aa, with N-terminal 10xHis-tagged tag, molecular weight 54.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection (PubMed:20545858, PubMed:20843794). Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate (PubMed:19846551, PubMed:20545858, PubMed:20843794). Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one (PubMed:19846551, PubMed:20545858, PubMed:20843794)

Pathway

Steroid metabolism; cholesterol degradation

Gene: cyp125  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: Cholest-4-en-3-one 26-monooxygenase; Cholest-4-en-3-one C26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming]; Cholesterol C26-monooxygenase; Cholesterol C26-monooxygenase [(25S)-3beta-hydroxycholest-5-en-26-oate forming]; Cytochrome P450 125; Steroid C27-monooxygenase
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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