Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD), Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 68.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD)

Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD) — Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Purity >85%.

SKU: BCRECP-003224 Categories: ,
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Product Specifications

Product SkuBCRECP-003224
Product DescriptionRecombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-569aa. [Accession | A0R4S9].
Uniprot No.A0R4S9
Gene NamesksdD
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-569aa
SpeciesMycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight68.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to.

Product Specifications

Recombinant Mycolicibacterium smegmatis 3-oxosteroid 1-dehydrogenase (ksdD) is a recombinant protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis), expressed in E.coli, covering amino acids 1-569aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 68.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to

Gene: ksdD  |  Organism: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)  |  Synonyms: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; 3-oxo-Delta(4)-steroid 1-dehydrogenase

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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