Recombinant Neisseria meningitidis serogroup B Factor H binding protein (fhbP), Neisseria meningitidis serogroup B (strain MC58), E.coli, N-terminal 6xHis-SUMO-tagged, 39.9kDa, purity >95% as determined by SDS-PAGE.

Recombinant Neisseria meningitidis serogroup B Factor H binding protein (fhbP)

Recombinant Neisseria meningitidis serogroup B Factor H binding protein (fhbP) — A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N. Purity >95%.

SKU: BCRECP-002735 Category:
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Product Specifications

Product SkuBCRECP-002735
Product DescriptionRecombinant Neisseria meningitidis serogroup B Factor H binding protein (fhbP) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 20-274aa. [Accession | Q9JXV4].
Uniprot No.Q9JXV4
Gene NamesfhbP
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region20-274aa
SpeciesNeisseria meningitidis serogroup B (strain MC58)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight39.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N.meningitidis. Binding of fH to the bacteria surface is independent of bacterial sialic acid moieties. fH binding affinity is high enough that it may sequester plasma fH, depleting its circulating levels and de-regulating complement in the host (Probable).

Biological Context

Subcellular Location: Cell outer membrane (Lipid-anchor); Secreted; Extracellular vesicle, bacterial extracellular vesicle

Product Specifications

Recombinant Neisseria meningitidis serogroup B Factor H binding protein (fhbP) is a recombinant protein from Neisseria meningitidis serogroup B (strain MC58), expressed in E.coli, covering amino acids 20-274aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 39.9kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N.meningitidis. Binding of fH to the bacteria surface is independent of bacterial sialic acid moieties (PubMed:16751403). fH binding affinity is high enough that it may sequester plasma fH, depleting its circulating levels and de-regulating complement in the host (Probable). This protein induces high levels of bactericidal antibodies in mice (PubMed:12642606, PubMed:15039331, PubMed:15664958, PubMed:21753121, PubMed:23133374)

Subcellular Location

Cell outer membrane (Lipid-anchor); Secreted; Extracellular vesicle, bacterial extracellular vesicle

Subunit

Binds to host factor H (fH from human) (PubMed:16751403, PubMed:16785547, PubMed:19225461, PubMed:23133374). Both fHbp beta-barrels contact Sushi domains 6 and 7 in fH (also called complement control protein domains, CCP). This interaction probably mimics the normal (carbohydrate-dependent) mode of fH recruitment, regulating fH activity. Sucrose octasulphate inhibits the fHbp-fH interaction (PubMed:16785547, PubMed:19225461, PubMed:23133374)

Gene: fhbP  |  Organism: Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58)  |  Synonyms: Genome-derived Neisseria antigen 1870; Lipoprotein 2086
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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