Recombinant Neisseria meningitidis serogroup B Neisserial heparin binding antigen (nhba), Neisseria meningitidis serogroup B (strain MC58), E.coli, C-terminal 6xHis-tagged, 55.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Neisseria meningitidis serogroup B Neisserial heparin binding antigen (nhba)

Recombinant Neisseria meningitidis serogroup B Neisserial heparin binding antigen (nhba) — [C2 fragment] Localizes to host mitochondria when applied to the apical side of human endothelial cell layers, where it induces production of reactive oxygen species which lead to increased permeab… Purit…

SKU: BCRECP-003872 Category:
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Product Specifications

Product SkuBCRECP-003872
Product DescriptionRecombinant Neisseria meningitidis serogroup B Neisserial heparin binding antigen (nhba) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 18-488aa. [Accession | Q7DD37].
Uniprot No.Q7DD37
Gene Namesnhba
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region18-488aa
SpeciesNeisseria meningitidis serogroup B (strain MC58)
Tag InfoC-terminal 6xHis-tagged
Molecular weight55.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

[C2 fragment] Localizes to host mitochondria when applied to the apical side of human endothelial cell layers, where it induces production of reactive oxygen species which lead to increased permeability of host endothelial cells. The C1 fragment (which lacks the first 14 residues of C2) does not have this effect. It is not known if this occurs during Neisseria infections.

Biological Context

Subcellular Location: Host mitochondrion

Product Specifications

Recombinant Neisseria meningitidis serogroup B Neisserial heparin binding antigen (nhba) is a recombinant protein from Neisseria meningitidis serogroup B (strain MC58), expressed in E.coli, covering amino acids 18-488aa, with C-terminal 6xHis-tagged tag, molecular weight 55.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

[C2 fragment] Localizes to host mitochondria when applied to the apical side of human endothelial cell layers, where it induces production of reactive oxygen species which lead to increased permeability of host endothelial cells. The C1 fragment (which lacks the first 14 residues of C2) does not have this effect. It is not known if this occurs during Neisseria infections

Subcellular Location

Host mitochondrion

Subunit

The C-terminal beta-barrel forms a monomer

Gene: nhba  |  Organism: Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58)  |  Synonyms: Genome-derived Neisseria antigen 2132
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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