Recombinant Neurospora crassa Chorismate synthase (aro-2), Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987), E.coli, C-terminal 6xHis-tagged, 46.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Neurospora crassa Chorismate synthase (aro-2)

Recombinant Neurospora crassa Chorismate synthase (aro-2) — Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the syn… Purity >90%.

SKU: BCRECP-002133 Categories: ,
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Product Specifications

Product SkuBCRECP-002133
Product DescriptionRecombinant Neurospora crassa Chorismate synthase (aro-2) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-432aa. [Accession | Q12640].
Uniprot No.Q12640
Gene Namesaro-2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-432aa
SpeciesNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Tag InfoC-terminal 6xHis-tagged
Molecular weight46.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the synthesis of the three aromatic amino acids phenylalanine, tyrosine and tryptophan. Acts also as a flavin reductase (FR) able to generate reduced flavin mononucleotide in the presence of NADPH.

Biological Context

Pathway: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7

Product Specifications

Recombinant Neurospora crassa Chorismate synthase (aro-2) is a recombinant protein from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987), expressed in E.coli, covering amino acids 1-432aa, with C-terminal 6xHis-tagged tag, molecular weight 46.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the synthesis of the three aromatic amino acids phenylalanine, tyrosine and tryptophan (PubMed:11034781, PubMed:11526120, PubMed:14668332, PubMed:17326665, PubMed:18279385, PubMed:4146266, PubMed:4155270, PubMed:7657620, PubMed:7819217, PubMed:7947820, PubMed:8971708). Acts also as a flavin reductase (FR) able to generate reduced flavin mononucleotide in the presence of NADPH (PubMed:11526120, PubMed:14668332, PubMed:18279385, PubMed:4146266, PubMed:4155270, PubMed:7947820, PubMed:8971708)

Subunit

Homotetramer

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7

Gene: aro-2  |  Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)  |  Synonyms: 5-enolpyruvylshikimate-3-phosphate phospholyase
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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