Recombinant Neurospora crassa Chorismate synthase (aro-2), Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987), E.coli, C-terminal 6xHis-tagged, 46.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Neurospora crassa Chorismate synthase (aro-2)

Recombinant Neurospora crassa Chorismate synthase (aro-2) — Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the syn… Purity >90%.

SKU: BCRECP-002133 Categories: ,
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Product Specifications

Product SkuBCRECP-002133
Product DescriptionRecombinant Neurospora crassa Chorismate synthase (aro-2) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-432aa. [Accession | Q12640].
Uniprot No.Q12640
Gene Namesaro-2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-432aa
SpeciesNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Tag InfoC-terminal 6xHis-tagged
Molecular weight46.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the synthesis of the three aromatic amino acids phenylalanine, tyrosine and tryptophan. Acts also as a flavin reductase (FR) able to generate reduced flavin mononucleotide in the presence of NADPH.

Biological Context

Pathway: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7

Product Specifications

Recombinant Neurospora crassa Chorismate synthase (aro-2) is a recombinant protein from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987), expressed in E.coli, covering amino acids 1-432aa, with C-terminal 6xHis-tagged tag, molecular weight 46.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Bifunctional chorismate synthase and flavin reductase that catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is the last common intermediate in the synthesis of the three aromatic amino acids phenylalanine, tyrosine and tryptophan (PubMed:11034781, PubMed:11526120, PubMed:14668332, PubMed:17326665, PubMed:18279385, PubMed:4146266, PubMed:4155270, PubMed:7657620, PubMed:7819217, PubMed:7947820, PubMed:8971708). Acts also as a flavin reductase (FR) able to generate reduced flavin mononucleotide in the presence of NADPH (PubMed:11526120, PubMed:14668332, PubMed:18279385, PubMed:4146266, PubMed:4155270, PubMed:7947820, PubMed:8971708)

Subunit

Homotetramer

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7

Gene: aro-2  |  Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)  |  Synonyms: 5-enolpyruvylshikimate-3-phosphate phospholyase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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