Recombinant Pig Saposin-B-Val (PSAP), Sus scrofa (Pig), Yeast, N-terminal 6xHis-Flag-tagged, 11.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pig Saposin-B-Val (PSAP)

Recombinant Pig Saposin-B-Val (PSAP) — Saposins are specific low-molecular mass non-enzymatic glycoproteins that act as activator proteins for lysosomal sphingolipid-degrading enzymes, facilitating the hydrolysis of sphingolipids by ext… Purity >90%.

SKU: BCRECP-004185 Category:
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Product Specifications

Product SkuBCRECP-004185
Product DescriptionRecombinant Pig Saposin-B-Val (PSAP) Protein is expressed from Yeast with N-terminal 6xHis-Flag-tagged. It contains 1-80aa. [Accession | P81405].
Uniprot No.P81405
Gene NamesPSAP
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-80aa
SpeciesSus scrofa (Pig)
Tag InfoN-terminal 6xHis-Flag-tagged
Molecular weight11.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Saposins are specific low-molecular mass non-enzymatic glycoproteins that act as activator proteins for lysosomal sphingolipid-degrading enzymes, facilitating the hydrolysis of sphingolipids by extracting lipid substrates from membranes and presenting them to their respective enzymes. They are derived from a common precursor protein, prosaposin, which is proteolytically cleaved to yield four homologous proteins (saposin A, B, C, and D), each with distinct but partially overlapping lipid and enzyme specificities.

Product Specifications

Recombinant Pig Saposin-B-Val (PSAP) is a recombinant protein from Sus scrofa (Pig), expressed in Yeast, covering amino acids 1-80aa, with N-terminal 6xHis-Flag-tagged tag, molecular weight 11.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Saposins are specific low-molecular mass non-enzymatic glycoproteins that act as activator proteins for lysosomal sphingolipid-degrading enzymes, facilitating the hydrolysis of sphingolipids by extracting lipid substrates from membranes and presenting them to their respective enzymes. They are derived from a common precursor protein, prosaposin, which is proteolytically cleaved to yield four homologous proteins (saposin A, B, C, and D), each with distinct but partially overlapping lipid and enzyme specificities

Subunit

Saposin-B is a homodimer. Interacts with GRN; facilitates lysosomal delivery of progranulin from the extracellular space and the biosynthetic pathway (By similarity)

Gene: PSAP  |  Organism: Sus scrofa

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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