Recombinant Pongo abelii NADPH oxidase 4 (NOX4) Protein, Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii), in vitro E.coli expression system, N-terminal 10xHis-tagged, 69.7kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Pongo abelii NADPH oxidase 4 (NOX4) Protein

Recombinant Pongo abelii NADPH oxidase 4 (NOX4) Protein — NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Purity >90%.

SKU: BCRECP-00231TM Categories: ,
Online Inquiry →

Product Specifications

Uniprot No.Q5R5C5
Gene NamesNOX4
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-578aa
SpeciesPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Tag InfoN-terminal 10xHis-tagged
Molecular weight69.7kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Can also catalyze to a smaller extent, the reduction of oxygen to superoxide. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity.

Biological Context

Subcellular Location: Cytoplasm; Endoplasmic reticulum membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cell junction, focal adhesion; Nucleus

Product Specifications

Recombinant Pongo abelii NADPH oxidase 4 (NOX4) Protein is a recombinant protein from Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii), expressed in in vitro E.coli expression system, covering amino acids 1-578aa, with N-terminal 10xHis-tagged tag, molecular weight 69.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (By similarity). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (By similarity). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (By similarity). May regulate insulin signaling cascade (By similarity). May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB (By similarity). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (By similarity). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (By similarity)

Subcellular Location

Cytoplasm; Endoplasmic reticulum membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Cell junction, focal adhesion; Nucleus

Subunit

Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with protein disulfide isomerase (By similarity). Interacts with PPP1R15A (By similarity). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (By similarity)

Gene: NOX4  |  Organism: Pongo abelii

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top