Recombinant Porcine parvovirus Capsid protein VP1, partial, Porcine parvovirus (strain NADL-2) (PPV), E.coli, N-terminal 6xHis-tagged, 26.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Porcine parvovirus Capsid protein VP1, partial

Recombinant Porcine parvovirus Capsid protein VP1, partial — Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which… Purity >90%.

SKU: BCRECP-003604 Categories: ,
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Product Specifications

Product SkuBCRECP-003604
Product DescriptionRecombinant Porcine parvovirus Capsid protein VP1, partial Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-207aa. [Accession | P18546].
Uniprot No.P18546
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-207aa
SpeciesPorcine parvovirus (strain NADL-2) (PPV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors.

Biological Context

Subcellular Location: Virion; Host nucleus

Product Specifications

Recombinant Porcine parvovirus Capsid protein VP1, partial is a recombinant protein from Porcine parvovirus (strain NADL-2) (PPV), expressed in E.coli, covering amino acids 1-207aa, with N-terminal 6xHis-tagged tag, molecular weight 26.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus (By similarity)

Subcellular Location

Virion; Host nucleus

Organism: Porcine parvovirus (strain NADL-2)  |  Synonyms: Coat protein VP1

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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