Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial, Pseudomonas aeruginosa (strain PAK), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 46.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial — Involved in pilus assembly, twitching motility and adhesion to host cells. Purity >90%.

SKU: BCRECP-004080 Category:
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Product Specifications

Product SkuBCRECP-004080
Product DescriptionRecombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 610-970aa. [Accession | S0HPF7].
Uniprot No.S0HPF7
Gene NamespilY1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region610-970aa
SpeciesPseudomonas aeruginosa (strain PAK)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight46.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in pilus assembly, twitching motility and adhesion to host cells. Primes type IV pili (T4P) assembly and is required for inclusion of minor pilins PilV, PilW and PilX to the surface pili. Stabilizes assembled pilus fibers likely by antagonizing retraction mediated by PilT.

Biological Context

Subcellular Location: Fimbrium; Membrane; Cytoplasm, cytosol

Product Specifications

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial is a recombinant protein from Pseudomonas aeruginosa (strain PAK), expressed in E.coli, covering amino acids 610-970aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 46.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in pilus assembly, twitching motility and adhesion to host cells. Primes type IV pili (T4P) assembly and is required for inclusion of minor pilins PilV, PilW and PilX to the surface pili (By similarity). Stabilizes assembled pilus fibers likely by antagonizing retraction mediated by PilT. Calcium-binding and calcium release by PilY1 seem to be essential for twitching motility and for regulation of pilus retraction dynamics of PilT. Adhesin for human tissue specifically recognizing a host receptor localized or enriched on basolateral epithelial cell surfaces. Binds host integrins in an calcium-dependent manner in vitro and this interaction may be employed by the bacterium to mediate host epithelial cell binding in vivo

Subcellular Location

Fimbrium; Membrane; Cytoplasm, cytosol

Subunit

Interacts (via C-terminal 532-1163) with host integrins alpha-V/beta-3 (ITGAV/ITGB3) and alpha-V/beta-5 (ITGAV/ITGB5)

Gene: pilY1  |  Organism: Pseudomonas aeruginosa (strain PAK)  |  Synonyms: Pilus-associated adhesin PilY
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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