Recombinant Pseudomonas putida 2-hydroxymuconate semialdehyde hydrolase (xylF), Pseudomonas putida (Arthrobacter siderocapsulatus), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 38.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Pseudomonas putida 2-hydroxymuconate semialdehyde hydrolase (xylF)

Recombinant Pseudomonas putida 2-hydroxymuconate semialdehyde hydrolase (xylF) — recombinant protein from Pseudomonas putida (Arthrobacter siderocapsulatus), expressed in E.coli. Purity >90%.

SKU: BCRECP-004757 Categories: ,
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Product Specifications

Product SkuBCRECP-004757
Product DescriptionRecombinant Pseudomonas putida 2-hydroxymuconate semialdehyde hydrolase (xylF) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-281aa. [Accession | P23106].
Uniprot No.P23106
Gene NamesxylF
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-281aa
SpeciesPseudomonas putida (Arthrobacter siderocapsulatus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight38.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate.

Biological Context

Pathway: Aromatic compound metabolism; benzoate degradation via hydroxylation

Product Specifications

Recombinant Pseudomonas putida 2-hydroxymuconate semialdehyde hydrolase (xylF) is a recombinant protein from Pseudomonas putida (Arthrobacter siderocapsulatus), expressed in E.coli, covering amino acids 1-281aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 38.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate

Pathway

Aromatic compound metabolism; benzoate degradation via hydroxylation

Gene: xylF  |  Organism: Pseudomonas putida  |  Synonyms: 2-hydroxymuconic semialdehyde hydrolase

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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