Product Specifications
Recombinant Pseudomonas putida Metapyrocatechase (xylE) — recombinant protein from Pseudomonas putida (Arthrobacter siderocapsulatus), expressed in E.coli. Purity >90%. Suitable for ELISA and Western Blot applications.
Recombinant Proteins for Research
Recombinant Pseudomonas putida Metapyrocatechase (xylE)
Recombinant Pseudomonas putida Metapyrocatechase (xylE) — recombinant protein from Pseudomonas putida (Arthrobacter siderocapsulatus), expressed in E.coli. Purity >90%.
Product Specifications
| Product Sku | BCRECP-004756 |
| Product Description | Recombinant Pseudomonas putida Metapyrocatechase (xylE) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 1-307aa. [Accession | P06622]. |
| Uniprot No. | P06622 |
| Gene Names | xylE |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-307aa |
| Species | Pseudomonas putida (Arthrobacter siderocapsulatus) |
| Tag Info | N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
| Molecular weight | 55.2kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
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Protein Biology
Subunit
Homotetramer
Pathway
Xenobiotic degradation; toluene degradation
Gene: xylE | Organism: Pseudomonas putida | Synonyms: CatO2ase; Catechol 2,3-dioxygenase
Key Publications
- Nakai C. et al. Complete nucleotide sequence of the metapyrocatechase gene on the TOL plasmid of Pseudomonas putida mt-2. — J. Biol. Chem. (1983)
- Zukowski M.M. et al. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene. — Proc. Natl. Acad. Sci. U.S.A. (1983)
- Zukowski M.M. et al. PubMed: CI-5U1983Q7T6J04 (MAY-)
- Neidle E.L. et al. Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily. — Eur. J. Biochem. (1992)
- Horn J.M. et al. DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism. — Mol. Microbiol. (1991)
- Kita A. et al. An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Ppseudomonas putida mt-2. — Structure (1999)
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Frequently Asked Questions
How do I order or inquire about this product?
Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.
What is the shipping and delivery time?
Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
How should this product be stored?
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
What quality controls are performed on your products?
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.