Recombinant Puumala virus Envelope glycoprotein (GP), partial, Puumala virus (strain Berkel), E.coli, C-terminal 6xHis-tagged, 36.3kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Puumala virus Envelope glycoprotein (GP), partial

Recombinant Puumala virus Envelope glycoprotein (GP), partial — Forms homotetramers with glycoprotein N at the surface of the virion. Purity >95%.

SKU: BCRECP-002887 Categories: ,
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Product Specifications

Product SkuBCRECP-002887
Product DescriptionRecombinant Puumala virus Envelope glycoprotein (GP) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-275aa. [Accession | P41264].
Uniprot No.P41264
Gene NamesGP
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-275aa
SpeciesPuumala virus (strain Berkel)
Tag InfoC-terminal 6xHis-tagged
Molecular weight36.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Forms homotetramers with glycoprotein N at the surface of the virion. Attaches the virion to host cell receptors including integrin ITGAV/ITGB3. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell surface; Host Golgi apparatus membrane (Single-pass type I membrane protein); Host endoplasmic reticulum membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Puumala virus Envelope glycoprotein (GP), partial is a recombinant protein from Puumala virus (strain Berkel), expressed in E.coli, covering amino acids 1-275aa, with C-terminal 6xHis-tagged tag, molecular weight 36.3kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms homotetramers with glycoprotein N at the surface of the virion. Attaches the virion to host cell receptors including integrin ITGAV/ITGB3. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell surface; Host Golgi apparatus membrane (Single-pass type I membrane protein); Host endoplasmic reticulum membrane (Single-pass type I membrane protein)

Subunit

Homodimer. Homotetramer; forms heterotetrameric Gn-Gc spikes in the pre-fusion conformation (By similarity). Homotrimer; forms homotrimer in the post-fusion conformation at acidic pH (By similarity). Interacts (via C-terminus) with the nucleoprotein (By similarity)

Gene: GP  |  Organism: Puumala virus (strain Berkel)  |  Synonyms: M polyprotein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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