Recombinant Rat ATP-sensitive inward rectifier potassium channel 10 (Kcnj10) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 6xHis-tagged, 46.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat ATP-sensitive inward rectifier potassium channel 10 (Kcnj10) Protein

Recombinant Rat ATP-sensitive inward rectifier potassium channel 10 (Kcnj10) Protein — May be responsible for potassium buffering action of glial cells in the brain. Purity >85%.

SKU: BCRECP-00353TM Category:
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Product Specifications

Uniprot No.P49655
Gene NamesKcnj10
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-379aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight46.6kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages.

Biological Context

Subcellular Location: Membrane (Multi-pass membrane protein); Basolateral cell membrane
Tissue Specificity: Predominantly expressed in the brain, including in glial cells of the cerebellum and forebrain. Expressed at lower levels in the kidney, and other peripheral tissues

Product Specifications

Recombinant Rat ATP-sensitive inward rectifier potassium channel 10 (Kcnj10) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-379aa, with N-terminal 6xHis-tagged tag, molecular weight 46.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May be responsible for potassium buffering action of glial cells in the brain (PubMed:7608203). Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (PubMed:7608203). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (By similarity)

Subcellular Location

Membrane (Multi-pass membrane protein); Basolateral cell membrane

Tissue Specificity

Predominantly expressed in the brain, including in glial cells of the cerebellum and forebrain. Expressed at lower levels in the kidney, and other peripheral tissues

Subunit

Homotetramer (PubMed:38355723). In kidney cells, it forms heteromeric channels with Kir5.1/KCNJ16; this interaction is required for KCNJ16 localization to the basolateral membrane. Interacts with MAGI1, alone and possibly as a heteromer with KCNJ16; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells (By similarity). Interacts with PATJ (By similarity)

Gene: Kcnj10  |  Organism: Rattus norvegicus  |  Synonyms: ATP-sensitive inward rectifier potassium channel KAB-2; BIR10; Brain-specific inwardly rectifying K(+) channel 1; Inward rectifier K(+) channel Kir4.1; Potassium channel, inwardly rectifying subfamily J member 10
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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