Recombinant Rat Claudin-1 (Cldn1) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 10xHis-tagged, 28.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Claudin-1 (Cldn1) Protein

Recombinant Rat Claudin-1 (Cldn1) Protein — Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. Purity >85%.

SKU: BCRECP-00078TM Category:
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Product Specifications

Uniprot No.P56745
Gene NamesCldn1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-211aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight28.9kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability.

Biological Context

Subcellular Location: Cell junction, tight junction; Cell membrane (Multi-pass membrane protein); Basolateral cell membrane
Tissue Specificity: Detected in epididymis (at protein level). Detected in testis and epididymis

Product Specifications

Recombinant Rat Claudin-1 (Cldn1) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-211aa, with N-terminal 10xHis-tagged tag, molecular weight 28.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (By similarity)

Subcellular Location

Cell junction, tight junction; Cell membrane (Multi-pass membrane protein); Basolateral cell membrane

Tissue Specificity

Detected in epididymis (at protein level). Detected in testis and epididymis

Subunit

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ. Interacts with OCLN, CD81, CLDN4, CLDN6 and CLDN9

Gene: Cldn1  |  Organism: Rattus norvegicus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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