Recombinant Rat Synaptotagmin-12 (Syt12) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 6xHis-tagged, 50.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Synaptotagmin-12 (Syt12) Protein

Recombinant Rat Synaptotagmin-12 (Syt12) Protein — Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release. Purity >85%.

SKU: BCRECP-00253TM Category:
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Product Specifications

Uniprot No.P97610
Gene NamesSyt12
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-421aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight50.7kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release. Unlike other synaptotagmins, it does not bind Ca(2+) or phospholipids. Essential for mossy-fiber long-term potentiation in the hippocampus.

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein)
Tissue Specificity: Expressed in the brain, specifically in neurons of the cerebellum, cortex, hippocampus, olfactory bulb, brainstem and spinal cord (at protein level)

Product Specifications

Recombinant Rat Synaptotagmin-12 (Syt12) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-421aa, with N-terminal 6xHis-tagged tag, molecular weight 50.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release but does not effect induced neurotransmitter release (PubMed:17190793). Unlike other synaptotagmins, it does not bind Ca(2+) or phospholipids (PubMed:17190793). Essential for mossy-fiber long-term potentiation in the hippocampus (By similarity)

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein)

Tissue Specificity

Expressed in the brain, specifically in neurons of the cerebellum, cortex, hippocampus, olfactory bulb, brainstem and spinal cord (at protein level)

Subunit

Homodimer (By similarity). Can also form heterodimers (By similarity). Interacts with SYT1 (PubMed:17190793)

Gene: Syt12  |  Organism: Rattus norvegicus  |  Synonyms: Synaptotagmin XII; Synaptotagmin-related gene 1 protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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