Recombinant Rat Tumor necrosis factor (Tnf), partial (Active) — recombinant protein, expressed in Yeast. Purity >95%.
| Product SKU | BCACP-000529 |
| Product Description | Recombinant Rat Tumor necrosis factor (Tnf) protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 80-235aa. [Accession | P16599]. |
| Uniprot No. | P16599 |
| Gene Names | Tnf |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | Yeast |
| Expression Region | 80-235aa |
| Species | Rattus norvegicus (Rat) |
| Tag Info | N-terminal 6xHis-tagged |
| Molecular weight | 19.2 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human FGFR3 at 2 μg/mL can bind Anti-FGFR3 recombinant antibody . The EC50 is 0.6767-0.8946 ng/mL. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a); Tumor necrosis factor, membrane form Alternative names: N-terminal fragment (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2 |
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Recombinant Rat Tumor necrosis factor (Tnf), partial (Active) is a recombinant protein, expressed in Yeast, covering amino acids 80-235aa, with N-terminal 6xHis-tagged tag, molecular weight 19.2 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications. Explore more TNF Family proteins →
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human FGFR3 at 2 μg/mL can bind Anti-FGFR3 recombinant antibody . The EC50 is 0.6767-0.8946 ng/mL.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
