Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (Active), Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), E.coli, N-terminal 6xHis-tagged, 49.7 kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (Active)

Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (Active) — May induce inflammasome responses in cultured cells and mice. Purity >85%.

SKU: BCACP-000057 Categories: , ,
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Product SKUBCACP-000057
Product DescriptionRecombinant SARS-CoV-2 Nucleoprotein (N) protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-419aa. [Accession | P0DTC9].
Uniprot No.P0DTC9
Gene NamesN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-419aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight49.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesN; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Function

May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation.

Biological Context

Subcellular Location: Virion; Host cytoplasm; Secreted; Host extracellular space

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (Active) is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in E.coli, covering amino acids 1-419aa, with N-terminal 6xHis-tagged tag, molecular weight 49.7 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed:39225180)

Subcellular Location

Virion; Host cytoplasm; Secreted; Host extracellular space

Subunit

Homodimer, homotetramer, homooligomer with RNA (PubMed:32654247, PubMed:38172120). Both monomeric and oligomeric forms interact with RNA. Interacts with protein M (By similarity). Interacts with protein E (By similarity). Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (PubMed:35044811). Interacts with host NLRP3 and ABHD8; these interactions disrupt the association between NLRP3 and ABHD8, increasing NLRP3 stability, ultimately leading to increased inflammasome activation (PubMed:34341353, PubMed:39225180). Interacts with host G3BP1; the interaction is enhanced by host HDAC6 which deacetylates the viral N protein and promotes N protein association with G3BP1, disrupting stress granule formation and facilitating viral replication (PubMed:39135075)

Gene: N  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: Nucleocapsid protein
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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