Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N), partial — May induce inflammasome responses in cultured cells and mice. Purity >90%.
| Product Sku | BCRECP-003480 |
| Product Description | Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) Protein is expressed from E.coli with C-terminal 10xHis-tagged. It contains 41-186aa. [Accession | P0DTC9]. |
| Uniprot No. | P0DTC9 |
| Gene Names | N |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 41-186aa |
| Species | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 17.4kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Microbiology |
May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N), partial — May induce inflammasome responses in cultured cells and mice. Purity >90%. Suitable for ELISA and Western Blot applications. Explore more Coronavirus Antigen proteins →
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed:39225180)
Virion; Host cytoplasm; Secreted; Host extracellular space
Homodimer, homotetramer, homooligomer with RNA (PubMed:32654247, PubMed:38172120). Both monomeric and oligomeric forms interact with RNA. Interacts with protein M (By similarity). Interacts with protein E (By similarity). Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (PubMed:35044811). Interacts with host NLRP3 and ABHD8; these interactions disrupt the association between NLRP3 and ABHD8, increasing NLRP3 stability, ultimately leading to increased inflammasome activation (PubMed:34341353, PubMed:39225180). Interacts with host G3BP1; the interaction is enhanced by host HDAC6 which deacetylates the viral N protein and promotes N protein association with G3BP1, disrupting stress granule formation and facilitating viral replication (PubMed:39135075)
Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.
Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
