Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active), Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Flag-tagged, 79.6 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active)

Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active) — Subunit of the fusion protein that is processed upon entry into the host cell. Purity >90%.

SKU: BCACP-000056 Categories: , , ,
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Product SKUBCACP-000056
Product DescriptionRecombinant SARS-CoV-2 Spike glycoprotein (S) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Flag-tagged. It contains 16-685aa. [Accession | P0DTC2].
Uniprot No.P0DTC2
Gene NamesS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region16-685aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoN-terminal 10xHis-tagged and C-terminal Flag-tagged
Molecular weight79.6 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind Biotinylated-SARS-CoV-2-S1-RBD , the EC50 is 5.087-7.050 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesS; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)

Function

Subunit of the fusion protein that is processed upon entry into the host cell. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains a viral fusion peptide that is unmasked after S2 cleavage.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active) is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in Mammalian cell, covering amino acids 16-685aa, with N-terminal 10xHis-tagged and C-terminal Flag-tagged tag, molecular weight 79.6 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Subunit of the fusion protein that is processed upon entry into the host cell. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains a viral fusion peptide that is unmasked after S2 cleavage. This cleavage can occur at the cell membrane by host TMPRSS2 or during endocytosis by host CSTL (PubMed:32703818, PubMed:34159616). In either case, this triggers an extensive and irreversible conformational change that leads to fusion of the viral envelope with the cellular cytoplasmic membrane, releasing viral genomic RNA into the host cell cytoplasm (PubMed:34561887). Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of the viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure and position the fusion peptide in close proximity to the C-terminal region of the ectodomain. Formation of this structure appears to promote apposition and subsequent fusion of viral and target cell membranes

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Binds to host ACE2 (PubMed:32075877, PubMed:32132184, PubMed:32155444, PubMed:32221306, PubMed:32225175, PubMed:32225176, PubMed:33607086). RBD also interacts with the N-linked glycan on 'Asn-90' of ACE2 (PubMed:33607086). Cleavage of S generates a polybasic C-terminal sequence on S1 that binds to host Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2) receptors (PubMed:33082293, PubMed:33082294). Interacts with host integrin alpha-5/beta-1 (ITGA5:ITGB1) and with ACE2 in complex with integrin alpha-5/beta-1 (ITGA5:ITGB1) (PubMed:33102950). May interact via cytoplasmic c-terminus with M protein (PubMed:33229438). May interact (via N-terminus) with host bilirubin and biliverdin, thereby preventing antibody binding to the SARS-CoV-2 spike NTD via an allosteric mechanism (PubMed:33888467). May interact with host LRRC15, thereby allowing attachment to host cells (PubMed:36735681)

Gene: S  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: E2; Peplomer protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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