Recombinant Shigella flexneri Invasin IpaD (IpaD), Shigella flexneri, E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 56.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Shigella flexneri Invasin IpaD (IpaD)

Recombinant Shigella flexneri Invasin IpaD (IpaD) — Required for bacterial invasion of host cells. Purity >90%.

SKU: BCRECP-003184 Category:
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Product Specifications

Product SkuBCRECP-003184
Product DescriptionRecombinant Shigella flexneri Invasin IpaD (IpaD) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 1-332aa. [Accession | P18013].
Uniprot No.P18013
Gene NamesipaD
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-332aa
SpeciesShigella flexneri
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight56.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via T3SS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Shigella flexneri Invasin IpaD (IpaD) is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-332aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 56.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via T3SS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal

Subcellular Location

Secreted

Gene: ipaD  |  Organism: Shigella flexneri  |  Synonyms: 36 kDa membrane antigen
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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