Recombinant Sindbis virus Structural polyprotein, partial Protein, Sindbis virus (SINV), in vitro E.coli expression system, N-terminal 10xHis-SUMO-tagged, 65.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Sindbis virus Structural polyprotein, partial Protein

Recombinant Sindbis virus Structural polyprotein, partial Protein — Class II viral fusion protein. Purity >85%.

SKU: BCRECP-00700TM Categories: ,
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Product Specifications

Uniprot No.P03316
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region807-1245aa
SpeciesSindbis virus (SINV)
Tag InfoN-terminal 10xHis-SUMO-tagged
Molecular weight65.9kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell via host VLDLR or LRP8/APOER2 and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Sindbis virus Structural polyprotein, partial Protein is a recombinant protein from Sindbis virus (SINV), expressed in in vitro E.coli expression system, covering amino acids 807-1245aa, with N-terminal 10xHis-SUMO-tagged tag, molecular weight 65.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion (PubMed:12573591, PubMed:21124457). After virus attachment to target cell via host VLDLR or LRP8/APOER2 and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits (PubMed:21124457, PubMed:34929721). This E1 trimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (PubMed:21124457). Efficient fusion requires the presence of cholesterol and sphingolipid in the target membrane (PubMed:10482600)

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Oligomer (By similarity). Interacts with spike glycoprotein E1 (PubMed:8892914). Interacts with spike glycoprotein E2 (PubMed:8892914)

Organism: Sindbis virus  |  Synonyms: p130
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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