Recombinant Staphylococcus aureus Sortase A (srtA), partial, Staphylococcus aureus (strain NCTC 8325), E.coli, N-terminal 6xHis-tagged, 24.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Sortase A (srtA), partial

Recombinant Staphylococcus aureus Sortase A (srtA), partial — Transpeptidase that anchors surface proteins to the cell wall. Purity >85%.

SKU: BCRECP-004483 Category:
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Product Specifications

Product SkuBCRECP-004483
Product DescriptionRecombinant Staphylococcus aureus Sortase A (srtA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 25-206aa. [Accession | Q2FV99].
Uniprot No.Q2FV99
Gene NamessrtA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-206aa
SpeciesStaphylococcus aureus (strain NCTC 8325)
Tag InfoN-terminal 6xHis-tagged
Molecular weight24.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Staphylococcus aureus Sortase A (srtA), partial is a recombinant protein from Staphylococcus aureus (strain NCTC 8325), expressed in E.coli, covering amino acids 25-206aa, with N-terminal 6xHis-tagged tag, molecular weight 24.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Transpeptidase that anchors surface proteins to the cell wall (PubMed:10427003, PubMed:10446208, PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall (PubMed:10446208, PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues (PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). Utilizes lipid II as the peptidoglycan substrate for the sorting reaction (PubMed:10446208, PubMed:11856734). Responsible for the display of important virulence factors (PubMed:14769030). Important for interactions with the host and host colonization during infection (PubMed:10805806, PubMed:14769030)

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Subunit

Monomer and homodimer; in equilibrium

Gene: srtA  |  Organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)  |  Synonyms: Surface protein sorting A
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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