Recombinant Staphylococcus aureus Sortase A (srtA), partial, Staphylococcus aureus (strain NCTC 8325), E.coli, N-terminal 6xHis-tagged, 24.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Sortase A (srtA), partial

Recombinant Staphylococcus aureus Sortase A (srtA), partial — Transpeptidase that anchors surface proteins to the cell wall. Purity >85%.

SKU: BCRECP-004483 Category:
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Product Specifications

Product SkuBCRECP-004483
Product DescriptionRecombinant Staphylococcus aureus Sortase A (srtA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 25-206aa. [Accession | Q2FV99].
Uniprot No.Q2FV99
Gene NamessrtA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-206aa
SpeciesStaphylococcus aureus (strain NCTC 8325)
Tag InfoN-terminal 6xHis-tagged
Molecular weight24.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Staphylococcus aureus Sortase A (srtA), partial is a recombinant protein from Staphylococcus aureus (strain NCTC 8325), expressed in E.coli, covering amino acids 25-206aa, with N-terminal 6xHis-tagged tag, molecular weight 24.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Transpeptidase that anchors surface proteins to the cell wall (PubMed:10427003, PubMed:10446208, PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall (PubMed:10446208, PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues (PubMed:10535938, PubMed:11714722, PubMed:14769030, PubMed:15247224). Utilizes lipid II as the peptidoglycan substrate for the sorting reaction (PubMed:10446208, PubMed:11856734). Responsible for the display of important virulence factors (PubMed:14769030). Important for interactions with the host and host colonization during infection (PubMed:10805806, PubMed:14769030)

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Subunit

Monomer and homodimer; in equilibrium

Gene: srtA  |  Organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)  |  Synonyms: Surface protein sorting A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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