Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiB (kaiB), Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), E.coli, C-terminal 6xHis-tagged, 18.3kDa, purity >95% as determined by SDS-PAGE.

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiB (kaiB)

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiB (kaiB) — A metamorphic protein which reversibly switches between an inactive tetrameric fold and a rare, thioredoxin-like monomeric fold (KaiB(fs)). Purity >95%.

SKU: BCRECP-003206 Category:
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Product Specifications

Product SkuBCRECP-003206
Product DescriptionRecombinant Synechococcus elongatus Circadian clock oscillator protein KaiB (kaiB) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-102aa. [Accession | Q79PF5].
Uniprot No.Q79PF5
Gene NameskaiB
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-102aa
SpeciesSynechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2)
Tag InfoC-terminal 6xHis-tagged
Molecular weight18.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

A metamorphic protein which reversibly switches between an inactive tetrameric fold and a rare, thioredoxin-like monomeric fold (KaiB(fs)). KaiB(fs) binds phospho-KaiC, KaiA and CikA. KaiA and CikA compete for binding to KaiB(fs), and KaiB(fs) and SasA compete for binding to KaiC, thus the clock oscillator and output signal pathway are tightly coupled.

Biological Context

Subcellular Location: Cytoplasm; Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiB (kaiB) is a recombinant protein from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), expressed in E.coli, covering amino acids 1-102aa, with C-terminal 6xHis-tagged tag, molecular weight 18.3kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

A metamorphic protein which reversibly switches between an inactive tetrameric fold and a rare, thioredoxin-like monomeric fold (KaiB(fs)). KaiB(fs) binds phospho-KaiC, KaiA and CikA. KaiA and CikA compete for binding to KaiB(fs), and KaiB(fs) and SasA compete for binding to KaiC, thus the clock oscillator and output signal pathway are tightly coupled

Subcellular Location

Cytoplasm; Cell membrane (Peripheral membrane protein)

Subunit

Undergoes a major conformational rearrangment; in the free state forms homotetramers with 2 dimers (PubMed:23796516). When bound to the CI domain of KaiC switches to a monomeric thioredoxin-fold (KaiB(fs)) (PubMed:26113641, PubMed:28302851, PubMed:28302852). Monomers, homodimers and homotetramers are detected in solution; at low concentrations only monomers are seen. In vitro forms KaiC(6):KaiB(1) and KaiC(6):KaiB(6) complexes (PubMed:24474762). Only associates with 'Ser-431'-phosphorylated KaiC (and not with doubly phosphorylated KaiC) (PubMed:17717528, PubMed:29892030). Complex formation between KaiB and KaiC is regulated by the phosphorylation state of KaiC and by an ATP hydrolysis-driven conformation change in the CI ring of KaiC; complex formation is slow. Slow complex formation is crucial for the timing of the circadian period (PubMed:29892030). In low resolution cryo-EM forms a KaiC(6):KaiB(6) complex (PubMed:23796516). The KaiABC complex composition changes during the circadian cycle to control KaiC phosphorylation. Complexes KaiC(6), KaiA(2-4):KaiC(6), KaiB(6):KaiC(6) and KaiC(6):KaiB(6):KaiA(12) are among the most important forms, many form cooperatively (PubMed:28302852, PubMed:34618577). The KaiB:KaiC complex is more prevalent at 16 hours (in the dark) than at 4 hours (in the light) in the circadian cycle (PubMed:10786837). The KaiA:KaiB complex is only found at 20-24 hours in the circadian cycle (subjective night) (PubMed:15347812). Binds to the CI domain of KaiC; SasA and KaiB compete to bind to the CI domain (PubMed:26113641, PubMed:28302851, PubMed:28302852)

Gene: kaiB  |  Organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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