Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiA (kaiA), Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), E.coli, C-terminal 6xHis-tagged, 39.6kDa, purity >95% as determined by SDS-PAGE.

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiA (kaiA)

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiA (kaiA) — KaiA binds oxidized quinones via its N-terminal PsR domain and is able to sense redox signals directly; quinone analog DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) blocks KaiA stimulatio… Purity >95%.

SKU: BCRECP-003205 Category:
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Product Specifications

Product SkuBCRECP-003205
Product DescriptionRecombinant Synechococcus elongatus Circadian clock oscillator protein KaiA (kaiA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-284aa. [Accession | Q79PF6].
Uniprot No.Q79PF6
Gene NameskaiA
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-284aa
SpeciesSynechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2)
Tag InfoC-terminal 6xHis-tagged
Molecular weight39.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

KaiA binds oxidized quinones via its N-terminal PsR domain and is able to sense redox signals directly; quinone analog DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) blocks KaiA stimulation of KaiC phosphorylation. The homodimer binds up to 8 quinones in the crystal structure, 3 in the PsR domain and 1 via the C-terminal helical bundle. Binding of oxidized quinone to the KaiA C-terminal domain reduces the phosphorylation of KaiC slightly; quinones may interact in a complex manner with KaiA to mediate clock input.

Product Specifications

Recombinant Synechococcus elongatus Circadian clock oscillator protein KaiA (kaiA) is a recombinant protein from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), expressed in E.coli, covering amino acids 1-284aa, with C-terminal 6xHis-tagged tag, molecular weight 39.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

KaiA binds oxidized quinones via its N-terminal PsR domain and is able to sense redox signals directly; quinone analog DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) blocks KaiA stimulation of KaiC phosphorylation (PubMed:20231482). The homodimer binds up to 8 quinones in the crystal structure, 3 in the PsR domain and 1 via the C-terminal helical bundle. Binding of oxidized quinone to the KaiA C-terminal domain reduces the phosphorylation of KaiC slightly; quinones may interact in a complex manner with KaiA to mediate clock input (PubMed:23020633)

Subunit

Homodimer (PubMed:15007067, PubMed:23020633, PubMed:26200123). The KaiABC complex composition changes during the circadian cycle to control KaiC phosphorylation. Complexes KaiC(6), KaiA(2-4):KaiC(6), KaiB(6):KaiC(6) and KaiC(6):KaiB(6):KaiA(12) are among the most important forms, many form cooperatively (PubMed:28302852, PubMed:34618577). The KaiA:KaiB complex is only found at 20-24 hours in the circadian cycle (subjective night) (PubMed:15347812). Binds to the C-terminal A-loop of KaiC via a coiled-coil structure (PubMed:26200123). KaiA and CikA compete for binding to KaiB(fs) (PubMed:26113641, PubMed:28302851). CikA copurifies with this protein in the clock complex (PubMed:17088557). Interacts with LdpA (PubMed:15775978)

Gene: kaiA  |  Organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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